RNA

Part:BBa_K1427000:Design

Designed by: Connor McBrine   Group: iGEM14_Tufts   (2014-10-09)


merRNA, c-di-GMP aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 398
    Illegal NheI site found at 426
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Teams should be mindful of their transcriptional start site when using the merRNA. The promoter should be constructed such that no additional nucleotides will be transcribed before the merRNA sequence, as this may cause misfolding.


Source

Bdellovibrio bacteriovorus

References